Effect of Extenders and Different Concentrations of Methanol on Motility Parameters of Goldfish (Carassius auratus gibelio) Spermatozoa after Short-Term Storage

Semen samples were collected from eight males of Goldfish (Carassius auratus gibelio) and cryopreserved using four extenders (Goldfish sperm extenders; GFSE 1– GFSE 4) supplemented with Methanol (MeOH) at concentration of 5%, 10% and 20%. Semen was diluted with ratio of 1:1 with extenders and frozen in liquid nitrogen vapor. Frozen sperms after 2 and 5 days were excluded from freezing. Experiment showed the highest motility duration and the most motility percentage of post-thawed sperms after 2 days for sperm frozen at extender GFSE 2 with the concentration of MeOH 10%(31.66±8.02 s and 16.66±3.51%; P<0.05), as well as the upmost mobility and the motility duration of post-thawed sperms after 5 days was observed for sperm frozen at extender GFSE 2 with the concentration of MeOH10% (22.66±3.78 s and 13±2.64%; P<0.05).